Accedi

University of Salzburg

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Markus Steiner¹, Andrea Harrer², Roland Lang³, Michael Schneider⁴, Fátima Ferreira⁵, Thomas Hawranek³, Martin Himly¹

¹Department of Molecular Biology, University of Salzburg, ²Department of Neurology, Paracelsus Medical University, ³Department of Dermatology, Paracelsus Medical University, ⁴Bühlmann Laboratories, ⁵Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg

Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.

Immunology and Infection

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Mario Wenger¹, Athanasios Bethanis¹, Litty Johnson¹, Lorenz Aglas¹

¹Department of Biosciences, University of Salzburg

Here we present the mediator release assay, using a rat basophilic leukemia cell line transfected with the human IgE receptor, to simulate the degranulation of effector cells typically observed in type 1 allergic reactions. This method investigates the biological activity of allergens in a highly sensitive, reproducible, and tailorable manner.