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Institut Cochin

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Laura Megrelis^1,2,3, Jérôme Delon^1,2,3

¹Inserm U1016, Institut Cochin, ²Cnrs, UMR8104, ³Université Paris Descartes, Sorbonne Paris Cité

Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.

Developmental Biology

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Cyrille Ramond^1,3, Antonio Bandeira², Claire Berthault³, Pablo Pereira³, Ana Cumano³, Odile Burlen-Defranoux³

¹Research Center Growth and Signaling, INSERM U845, Institut Cochin, ²Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy, University of Pierre & Marie Curie, ³Unit for Lymphopoiesis, Immunology Department, INSERM U668, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur

This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.