Accedi

Max Planck Institute for Molecular Biomedicine

3 ARTICLES PUBLISHED IN JoVE

Biology

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

Miao Zhang¹, Hans R. Schöler^1,2, Boris Greber¹

¹Max Planck Institute for Molecular Biomedicine, ²Medical Faculty, University of Münster

Protocols for neuronal differentiation of pluripotent human stem cells (hPSCs) are often time-consuming and require substantial cell culture skills. Here, we have adapted a small molecule-based differentiation procedure to a multititre plate format, allowing simple, rapid, and efficient generation of human neurons in a controlled manner.

JoVE Journal

Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT

Bowen Shi¹, Huimin Lin¹, Miao Zhang², Wei Lu³, Ying Qu⁴, Huan Zhang¹

¹Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, ²Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, ³GE Healthcare China, ⁴Department of Surgery, Cedars-Sinai Medical Center

This protocol describes the value of dual energy CT and PET/CT imaging methods in tumor imaging and efficacy evaluation. This article demonstrates the research methods and results acquired by dual energy CT and PET/CT to evaluate the gene regulation and targeted treatment of gastric cancer peritoneal metastasis.

Genetics

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

Clemens B. Hug¹, Juan M. Vaquerizas¹

¹Max Planck Institute for Molecular Biomedicine

This work describes a protocol for the generation of high resolution in situ Hi-C libraries from tightly staged pre-gastrulation Drosophila melanogaster embryos.