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Texas Children's Hospital

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
Jennifer Selever 1, Jian-Qiang Kong 2, Benjamin R. Arenkiel 3,4
1Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience, Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital

Here we describe a rapid and simple method to image fluorescently labeled cells in semi-thick brain slices. By fixing, slicing, and optically clearing brain tissue we describe how standard epifluorescent or confocal imaging can be used to visualize individual cells and neuronal networks within intact nervous tissue.

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Neuroscience

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue
Kevin Ung 1, Benjamin R. Arenkiel 1,2,3
1Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), 2Department of Neuroscience, Baylor College of Medicine (BCM), 3Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital

The development of optogenetics now provides the means to precisely stimulate genetically defined neurons and circuits, both in vitro and in vivo. Here we describe the assembly and implantation of a fiber optic for chronic photostimulation of brain tissue.

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Neuroscience

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice
Gary Liu *1,2, Jay M. Patel *2,3, Burak Tepe 1, Cynthia K. McClard 2,4, Jessica Swanson 4, Kathleen B. Quast 4, Benjamin R. Arenkiel 1,3,4,5
1Program in Developmental Biology, Baylor College of Medicine, 2Medical Scientist Training Program, Baylor College of Medicine, 3Department of Neuroscience, Baylor College of Medicine, 4Department of Molecular and Human Genetics, Baylor College of Medicine, 5Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital

Here, we train mice on an associative learning task to test odor discrimination. This protocol also allows for studies on learning-induced structural changes in the brain.

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Neuroscience

Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing
Brandon T. Pekarek 1, Patrick J. Hunt 1,2, Benjamin D. W. Belfort 1,2, Gary Liu 2, Benjamin R. Arenkiel 1,3
1Department of Genetics and Genomics, Baylor College of Medicine, 2Medical Scientist Training Program, Baylor College of Medicine, 3Department of Neuroscience, Baylor College of Medicine

Neuronal dendritic morphology often underlies function. Indeed, many disease processes that affect the development of neurons manifest with a morphological phenotype. This protocol describes a simple and powerful method for analyzing intact dendritic arbors and their associated spines.

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