Quantitative High-throughput Single-cell Cytotoxicity Assay For T CellsIvan Liadi 1, Jason Roszik 2, Gabrielle Romain 1, Laurence J.N. Cooper 2, Navin Varadarajan 1
1Department of Chemical and Biomolecular Engineering, University of Houston , 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center
We describe a single-cell high-throughput assay to measure cytotoxicity of T cells when incubated with tumor target cells. This method employs a dense, elastomeric array of sub-nanoliter wells (~100,000 wells/array) to spatially confine the T cells and target cells at defined ratios and is coupled to fluorescence microscopy to monitor effector-target conjugation and subsequent apoptosis.
