Accedi

National Institute on Drug Abuse (NIDA)

1 ARTICLES PUBLISHED IN JoVE

Biology

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

Jaime M. Ross^1,2

¹Department of Neuroscience, Karolinska Institutet, ²National Institute on Drug Abuse (NIDA)

The cytochrome c oxidase/sodium dehydrogenase (COX/SDH) double-labeling method allows for direct visualization of mitochondrial respiratory enzyme deficiencies in fresh-frozen tissue sections. This is a straightforward histochemical technique and is useful in investigating mitochondrial diseases, aging, and aging-related disorders.