Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D EnvironmentsXiaoting Meng *1, Wenfei Li *2,3, Fraser Young 1, Runchi Gao 3, Laura Chalmers 3, Min Zhao 3, Bing Song 1
1School of Dentistry, Cardiff Institute of Tissue Engineering & Repair, Cardiff University , 2Shandong Qianfoshan Hospital, Shandong University School of Medicine, 3Dermatology and Ophthalmology Research, Institute for Regenerative Cures, University of California at Davis
This protocol demonstrates methods used to establish 2D and 3D environments in custom-designed electrotactic chambers, which can track cells in vivo/ex vivo using time-lapse recording at the single cell level, in order to investigate galvanotaxis/electrotaxis and other cellular responses to direct current (DC) electric fields (EFs).
