An Efficient and Flexible Cell Aggregation Method for 3D Spheroid ProductionSarah M. Maritan *1,2, Eric Y. Lian *1,2, Lois M. Mulligan 1,2
1Division of Cancer Biology and Genetics, Queen's University Cancer Research Institute, 2Department of Pathology and Molecular Medicine, Queen's University
Here, we describe a rapid and flexible protocol for the formation of 3D cell spheroids through cell aggregation. This is easily adapted to multiple cell types and is suitable for use in a variety of applications including cell migration, invasion, or anoikis assays, and for imaging and quantifying cell-matrix interactions.
