Accedi

Northshore University Healthsystem

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Jack K. Phillips¹, Sydney A. Sherman^1,2, Sevan R. Oungoulian³, John D. Finan¹

¹Department of Neurosurgery, NorthShore University HealthSystem, ²Midwestern University/Chicago College of Osteopathic Medicine, ³Independent Contractor

Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.

Cancer Research

Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors

Alexander P. Glaser¹, Daniele Procissi², Yanni Yu³, Joshua J. Meeks³

¹Department of Surgery, Division of Urology, NorthShore University HealthSystem, ²Department of Radiology, Northwestern University Feinberg School of Medicine, ³Department of Urology, Northwestern University Feinberg School of Medicine

Murine bladder tumors are induced with the N-butyl-N-(4-hydroxybutyl) nitrosamine carcinogen (BBN). Bladder tumor generation is heterogeneous; therefore, an accurate assessment of tumor burden is needed before randomization to experimental treatment. Here we present a fast, reliable MRI protocol to assess tumor size and stage.