Accedi

Northeast Forestry University

2 ARTICLES PUBLISHED IN JoVE

Biology

Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

Sheldon Lawrence II^1,2,3, Quiying Pang^2,3,4, Wenwen Kong^2,3, Sixue Chen^1,2,3,5

¹Plant Molecular and Cellular Biology Program, University of Florida, ²Department of Biology, University of Florida, ³Genetics Institute (UFGI), University of Florida, ⁴Alkali Soil Natural Environmental Science Center, Northeast Forestry University, ⁵Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida

This protocol describes a method of preparing enriched stomatal guard cells that is useful for physiological and other biological studies.

Biology

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Jinxi Yuan¹, Jie Zhang¹, Yan Zhang¹, WuYun QiQiGe¹, Wei Liu², Shanchun Yan¹, Guirong Wang²

¹Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, ²Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.