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Kitasato university

2 ARTICLES PUBLISHED IN JoVE

Biology

Measurement of Survival Time in Brachionus Rotifers: Synchronization of Maternal Conditions

Gen Kaneko^1,4, Tatsuki Yoshinaga², Kristin E. Gribble³, David M. Welch³, Hideki Ushio¹

¹Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, ²School of Marine Biosciences, Department of Marine Biosciences, Kitasato University, ³Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, ⁴School of Arts and Sciences, University of Houston-Victoria

Rotifers are microscopic zooplankton used as models in ecotoxicological and aging studies. Here we provide a protocol for powerful and reproducible measurement of survival time in Brachionus rotifers. Synchronization of culture conditions over several generations is of particular importance because maternal condition affects life history of offspring.

Environment

Visualization of Low-Level Gamma Radiation Sources Using a Low-Cost, High-Sensitivity, Omnidirectional Compton Camera

Hiroshi Muraishi¹, Ryoji Enomoto², Hideaki Katagiri³, Mika Kagaya⁴, Takara Watanabe⁵, Naofumi Narita³, Daisuke Kano⁵

¹School of Allied Health Sciences, Kitasato University, ²Institute for Cosmic Ray Research, University of Tokyo, ³College of Science, Ibaraki University, ⁴National Institute of Technology, Sendai College, ⁵National Cancer Center Hospital East

We present experimental protocols for visualizing various low-level gamma radiation sources in the ambient environment using a low-cost, high-sensitivity, omnidirectional, gamma-ray imaging Compton camera.