Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes ResearchAnna U. Eriksson *1, Christoffer Svensson *1, Andreas Hörnblad 1, Abbas Cheddad 1, Elena Kostromina 1, Maria Eriksson 1, Nils Norlin 1, Antonello Pileggi 2, James Sharpe 3, Fredrik Georgsson 4, Tomas Alanentalo 1, Ulf Ahlgren 1
1Umeå Centre for Molecular Medicine, Umeå University, 2Cell Transplant Center, Diabetes Research Institute, University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science, Umeå University
We describe the adaptation of optical projection tomography (OPT)1 to imaging in the near infrared spectrum, and the implementation of a number of computational tools. These protocols enable assessments of pancreatic β-cell mass (BCM) in larger specimens, increase the multichannel capacity of the technique and increase the quality of OPT data.
