Accedi

Academic Medical Center

4 ARTICLES PUBLISHED IN JoVE

Medicine

Human Dupuytren's Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions

Sofia Karkampouna¹, Peter Kloen², Miryam C. Obdeijn³, Scott M. Riester⁴, Andre J. van Wijnen^4,5, Marianna Kruithof-de Julio^1,6

¹Department of Molecular Cell Biology, Cancer Genomics Centre and Centre for Biomedical Genetics, Leiden University Medical Center, ²Department of Orthopedic Surgery, Academic Medical Center, ³Department of Plastic, Reconstructive and Hand Surgery, Academic Medical Center, ⁴Department of Orthopedic Surgery, Mayo Clinic, ⁵Department of Biochemistry and Molecular Biology, Mayo Clinic, ⁶Department of Dermatology, Leiden University Medical Center

Dupuytren’s disease (DD) is a fibroproliferative disease of the palm of the hand. Here, we present a protocol to culture resection specimens from DD in a three-dimensional (3D) culture system. Such short-term culture system allows preservation of the 3D structure and molecular properties of the fibrotic tissue.

Medicine

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Peter G. Wagstaff¹, Abel Swaan², Alexandre Ingels¹, Patricia J. Zondervan¹, Otto M. van Delden³, Dirk J. Faber², Ton G. van Leeuwen², Jean J. de la Rosette¹, Daniel M. de Bruin^1,2, M. Pilar Laguna Pes¹

¹Department of Urology, Academic Medical Center, ²Department of Biomedical Engineering and Physics, Academic Medical Center, ³Department of Radiology, Academic Medical Center

Optical coherence tomography (OCT) is a high resolution imaging technique that allows analysis of tissue specific optical properties providing the means for tissue differentiation. We developed needle based OCT, providing real-time imaging combined with on-the-spot tumor differentiation. This publication describes a method for percutaneous, needle based OCT of renal masses.

Bioengineering

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Sandra Van de Wiel¹, Maarten Merkx^2,3, Stan Van de Graaf¹

¹Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, ²Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), ³Department of Biomedical Engineering, Eindhoven University of Technology

We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.

Cancer Research

Laparoscopic Pancreatoduodenectomy With Modified Blumgart Pancreaticojejunostomy

Matteo De Pastena^1,2, Jony van Hilst¹, Thijs de Rooij¹, Olivier R Busch¹, Michael F Gerhards³, Sebastiaan Festen³, Marc G Besselink¹

¹Department of Surgery, Cancer Center Amsterdam, Academic Medical Center, ²General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona, ³Department of Surgery, OLVG

Laparoscopic pancreatoduodenctomy (LPD) may offer advantages over open pancreatoduodenectomy, including early postoperative mobilization, less delayed gastric emptying and a shorter hospital stay. However, LPD is technically challenging and not well-standardized, especially regarding the pancreatic anastomosis. We describe a standardized technique for the pancreatic anastomosis during LPD: modified Blumgart pancreaticojejunostomy.