Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic SalmonMarta S. Silva 1,2, Thea Stewart 4, Heidi Amlund 1,3, Jens J. Sloth 1,3, Pedro Araujo 1, Erik-Jan Lock 1, Christer Hogstrand 4, Robin Ørnsrud 1, Rune Waagbø 1,2, Antony Jesu Prabhu 1
1Institute of Marine Research, 2Department of Biological Sciences, University of Bergen, 3National Food Institute, Technical University of Denmark, 4Metal metabolism group, Nutritional Sciences Division, King's College London
This article explains in detail a systematic approach to assess micro-mineral availability in Atlantic salmon. The methodology includes tools and models with increasing biological complexity: (1) chemical speciation analysis, (2) in vitro solubility, (3) uptake studies in cell lines, and (4) in vivo fish studies.
