Accedi

Hubrecht Institute

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
Sina Bartfeld 1, Hans Clevers 1
1Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre Utrecht

Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.

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Medicine

Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
Sylvia F. Boj 1, Annelotte M. Vonk 2, Marvin Statia 1, Jinyi Su 1, Johanna F. Dekkers 3, Robert R. G. Vries 1, Jeffrey M. Beekman 2, Hans Clevers 1,4
1Foundation Hubrecht Organoid Technology, 2Department of Pediatric Pulmonology, Regenerative Medicine Centre Utrecht, Wilhelmina Children's Hospital, University Medical Centre Utrecht, 3Department of Stem Cells and Cancer, Walter and Eliza Hall Institute of Medical Research, 4Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre Utrecht

This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.

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Biology

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
Marie Bannier-Hélaouët 1,2, Maarten H. Geurts 1,2, Jeroen Korving 1, Harry Begthel 1, Hans Clevers 1,2
1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), University Medical Center Utrecht, 2Oncode Institute, Hubrecht Institute

This protocol describes how to establish, maintain, genetically modify, differentiate, functionally characterize, and transplant lacrimal gland organoids derived from primary mouse and human tissue.

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