Accedi

Centre de recherche du CHU Sainte-Justine

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

¹Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Université Laval, ²Centre de recherche du CHU Sainte-Justine

Here, we provide an easy, low-cost, and time-efficient protocol to chemically fix primate brain tissue with acrolein fixative, allowing for long-term preservation that is compatible with pre-embedding immunohistochemistry for transmission electron microscopy.

Neuroscience

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Lara Eid^1,2, Mathieu Lachance¹, Gilles Hickson^1,3, Elsa Rossignol^1,2,4

¹Centre de recherche du CHU Sainte-Justine, ²Department of Neuroscience, Université de Montréal, ³Department of pathology and cellular biology, Université de Montréal, ⁴Department of Pediatrics, Université de Montréal

Here, we provide a low-cost and reliable method to generate electroporated brain organotypic slice cultures from mouse embryos suitable for confocal microscopy and live-imaging techniques.