Accedi

University of the Basque Country (UPV/EHU)

2 ARTICLES PUBLISHED IN JoVE

Biology

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

Brendan M. O'Leary¹, Arantza Rico², Sarah McCraw¹, Helen N. Fones³, Gail M. Preston¹

¹Department of Plant Sciences, University of Oxford, ²School of Education of Vitoria-Gasteiz, University of the Basque Country (UPV/EHU), ³Biosciences, University of Exeter

This protocol details the optimized extraction of apoplast washing fluid from plant leaves, using French bean plants (Phaseolus vulgaris) as a model example.

Neuroscience

Histological Examination of Mitochondrial Morphology in a Parkinson's Disease Model

Dalila Ciceri^*1, Lierni Gregorio-Zabala^*1, Xabier Llama-Pino¹, Begüm Kurt¹, Jon Olano-Bringas¹, Patricia Villegas-Zafra¹, Nora Bengoa-Vergniory^1,2,3,4

¹Achucarro Basque Center for Neuroscience, ²Department of Neuroscience, University of the Basque Country (UPV/EHU), ³Ikerbasque - Basque Foundation for Science, ⁴Oxford Parkinson’s Disease Centre and Department of Physiology, Anatomy and Genetics, University of Oxford

This study presents a method to analyze the morphology of mitochondria based on immunostaining and image analysis in mouse brain tissue in situ. It also describes how this allows one to detect changes in mitochondrial morphology induced by protein aggregation in Parkinson's disease models.