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1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Xianming Yang¹, Pengjun Xu², Robert I. Graham³, He Yuan¹, Kongming Wu¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, ²Tobacco Research Institute, Chinese Academy of Agricultural Sciences, ³Crop and Environment Sciences, Harper Adams University

Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.