Accedi

California Northstate University

2 ARTICLES PUBLISHED IN JoVE

Environment

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

Jodi R. Schilz¹, K. J. Reddy², Sreejayan Nair³, Thomas E. Johnson⁴, Ronald B. Tjalkens⁵, Kem P. Krueger³, Suzanne Clark⁶

¹Division of Physical Therapy, Department of Orthopedics & Rehabilitation, University of New Mexico, ²Department of Ecosystem Science and Management, University of Wyoming, ³School of Pharmacy, University of Wyoming, ⁴Department of Environmental and Radiological Health Sciences, Colorado State University, ⁵Center for Environmental Medicine, Colorado State University, ⁶College of Pharmacy, California Northstate University

Production bleed water (PBW) was treated with cupric oxide nanoparticles (CuO-NPs) and cellular toxicity was assessed in cultured human cells. The goal of this protocol was to integrate the native environmental sample into a cell culture format assessing the changes in toxicity due to CuO-NP treatment.

Biology

Using Caco-2 Cells to Study Lipid Transport by the Intestine

Andromeda M. Nauli¹, Judy D. Whittimore²

¹Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, ²Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University

Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.