An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corrolesGrace Y. Tang 1, Melanie A. Pribisko 1, Ryan K. Henning 1, Punnajit Lim 2, John Termini 2, Harry B. Gray 1, Robert H. Grubbs 1
1Division of Chemistry and Chemical Engineering, California Institute of Technology, 2Department of Molecular Medicine, Beckman Research Institute of the City of Hope
Gallium(III) 5,10,15-(tris)pentafluorophenylcorrole and its freebase analogue exhibit low micromolar cell cytotoxicity. This manuscript describes an RNA transcription reaction, imaging RNA with an ethidium bromide-stained gel, and quantifying RNA with UV-Vis spectroscopy, in order to assess transcription inhibition by corroles and demonstrates a straightforward method of evaluating anticancer candidate properties.
