JoVE | Peer Reviewed Scientific Video Journal

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International Expert Consensus and Recommendations for Neonatal Pneumothorax Ultrasound Diagnosis and Ultrasound-guided Thoracentesis Procedure

Jing Liu^1,2, Dalibor Kurepa³, Francesco Feletti^4,5, Almudena Alonso-Ojembarrena⁶, Jovan Lovrenski⁷, Roberto Copetti⁸, Erich Sorantin⁹, Javier Rodriguez-Fanjul¹⁰, Karishma Katti³, Andrea Aliverti⁴, Huayan Zhang^11,12, Misun Hwang¹³, Tsu F. Yeh¹⁴, Cai-Bao Hu¹⁵, Xing Feng¹⁶, Ru-Xin Qiu^1,2, Jing-Han Chi¹⁷, Li-Li Shang¹⁸, Guo-Rong Lyu¹⁹, Shao-Zheng He²⁰, Yan-Fen Chai²¹, Zhan-Jun Qiu²², Hai-Ying Cao^2,23, Yue-Qiao Gao^1,2, Xiao-Ling Ren^1,2, Guo Guo^1,24, Li Zhang^1,2, Ying Liu^1,2, Wei Fu^1,2, Zu-Lin Lu^1,2, Hong-Lei Li^1,2

¹Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, ²The National Neonatal Lung Ultrasound Training Base, ³Division of Neonatal-Perinatal Medicine, Cohen Children's Medical Center, ⁴Department of Electronics, Information and Bioengineering, Politecnico di Milano, ⁵Dipartimento di Diagnostica per Immagini, Ausl della Romagna, S. Maria delle Croci Hospital, ⁶Neonatal Intensive Care Unit, Puerta del Mar University Hospital, ⁷Faculty of Medicine, University of Novi Sad, Serbia, Institute for Children and Adolescents Health Care of Vojvodina, ⁸Emergency Department, University Hospital of Cattinara, ⁹Division of Pediatric Radiology, Department of Radiology, Medical University Graz, ¹⁰Pediatric Intensive Care Unit, Pediatric Service Hospital Joan XXIII Tarragona, University Rovira i Virgil, ¹¹Center for Newborn Care, Guangzhou Women and Children's Medical Center, ¹²Division of Neonatology, Children's Hospital of Philadelphia, ¹³Section of Neonatal Imaging, Department of Radiology, Children's Hospital of Philadelphia, ¹⁴Maternal Child Health Research institute, Taipei Medical University and China Medical University, ¹⁵Intensive Care Unit, Zhejiang Hospital, ¹⁶Department of Neonatology, Children's Hospital of Soochow University, ¹⁷Department of Neonatology and NICU, Bayi Children's Hospital Affiliated to the Seventh Medical Center of Chinese PLA General Hospital, ¹⁸Intensive Care Unit, Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, ¹⁹Collaborative Innovation Center for Maternal and Infant Health Service Application Technology, Quanzhou Medical College, ²⁰Department of Ultrasound, Second Affiliated Hospital of Fujian Medical University, ²¹Department of Emergency Medicine, Tianjin Medical University General Hospital, ²²Department of Emergency and Critical Care Medicine, Affiliated Hospital of Traditional Chinese Medicine, ²³Department of Ultrasound, GE Healthcare, ²⁴The Neonatal Intensive Care Unit, Fifth Medical Center of Chinese PLA General Hospital

Pneumothorax is a common emergency and critical disease in newborn infants that needs rapid, clear diagnosis and timely treatment. Diagnosis and treatment based on chest X-rays are associated with delayed management and radiation damage. Lung ultrasound (US) provides useful guidance for rapid, accurate diagnosis and the precise thoracentesis of pneumothorax.

Immunology and Infection

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

Lichen Zhang¹, Rong Huang¹, Liaoxun Lu¹, Rui Fu¹, Guo Guo¹, Yanrong Gu¹, Zhuangzhuang Liu¹, Le He¹, Marie Malissen², Yinming Liang¹

¹The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, ²Centre d’Immunologie de Marseille-Luminy, Aix Marseille Université

This protocol uses fluorescent reporters and cell sorting to simplify knock-in experiments in macrophage and T cell lines. Two plasmids are used for these simplified knock-in experiments, namely a CRISPR/Cas9- and DsRed2-expressing plasmid and a homologous recombination donor plasmid expressing EBFP2, which is permanently integrated at the Rosa26 locus in immune cells.

Medicine

Mouse Wound Models and Preparation of Single-Cell Suspensions

Yuanyuan Liu¹, Mingwang Zhang², Kai Luo³, Yurui Zou⁴, Rongya Yang⁵

¹Medical School of Chinese People's Liberation Army, ²Department of Dermatology, Southwest Hospital, Army Medical University, ³Biomedical Treatment Center, the Seventh Medical Center, Chinese PLA General Hospital, ⁴The 83th Affiliated Hospital, Xinxiang Medical University, ⁵Department of Dermatology, the Seventh Medical Center, Chinese PLA General Hospital

This study presents two crucial experimental techniques: the construction of a murine wound model for assessing wound closure and the preparation of single-cell suspensions from murine skin to preserve cellular viability.

Xinxiang medical university

3 ARTICLES PUBLISHED IN JoVE

JoVE Core

Immunology and Infection

Medicine