Accedi

Nanchang University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

Cheng Qin^*1,2, Yijiang Bai^*1,2, Zhen Zeng^1,2, Liao Wang^1,2, Zhiwen Luo^1,2, Shunqi Wang^1,3, Suqi Zou^1,3

¹Institute of Life Science, Nanchang University, ²Queen Mary School, Medical Department, Nanchang University, ³School of Life Science, Nanchang University

Here, we present a protocol to improve paraffin sectioning. This method combines cutting and floating using a simple thermostatic chamber to avoid the transfer process required by the conventional method. As a result, the efficiency and the number of intact paraffin sections were greatly improved.

Biochemistry

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels

Yue Zhao¹, Mai Hu², Yiwen Gong², Yihao Zhao², Zhiwei Shen²

¹The First Affiliated Hospital of Nanchang University, ²The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University

This protocol presents three fast and simple preparation methods that use environmental conditions to trigger the self-assembly of peptides into hydrogels. Additionally, the characterization of peptide hydrogels is described, demonstrating that mechanically stable peptide hydrogels can be formed under these straightforward conditions.