Accedi

Friedrich Miescher Institute for Biomedical Research

2 ARTICLES PUBLISHED IN JoVE

Behavior

Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment

Marcus Leinweber^*1,2, Pawel Zmarz^*1,2, Peter Buchmann³, Paul Argast¹, Mark Hübener², Tobias Bonhoeffer², Georg B. Keller^1,2

¹Friedrich Miescher Institute for Biomedical Research, ²Max Planck Institute of Neurobiology, ³Department of Biosystems Science and Engineering, ETH Zurich

Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.

Biochemistry

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Patricia E. Nigg^1,2, Jovan Pavlovic¹

¹Institute for Medical Virology, University of Zurich, ²Friedrich Miescher Institute for Biomedical Research

This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.