Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian CellsNathaniel W Holton 1, Joel F Andrews 1, Natalie R Gassman 1
1Department of Oncologic Sciences, University of South Alabama Mitchell Cancer Institute
Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.
