Accedi

Noble Research Institute

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

Peilin Zheng^*1,2, Grant Bertolet^*1,2,3, Yuhui Chen^1,2, Shengjian Huang^1,2, Dongfang Liu^1,2,3

¹Center for Human Immunobiology, Texas Children's Hospital, ²Department of Pediatrics, Baylor College of Medicine, ³Department of Pathology and Immunology, Baylor College of Medicine

We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.

JoVE Journal

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Yuhui Chen¹, Xianfu Wang², Shunfei Lu³, Hongcheng Wang², Shibo Li², Rujin Chen¹

¹Laboratory of Plant Genetics and Development, Noble Research Institute, ²Genetics Laboratory, University of Oklahoma Health Science Center, ³Medicine and Health School, Li Shui University

This protocol provides experimental steps and information about reagents, equipment, and analysis tools for researchers who are interested in carrying out whole genome array-based comparative genomic hybridization (CGH) analysis of copy number variations in plants.