Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery MethodJonathan T. Ting *1, Brian R. Lee *1, Peter Chong 1, Gilberto Soler-Llavina 1, Charles Cobbs 2, Christof Koch 1, Hongkui Zeng 1, Ed Lein 1
1Cell Types Program, Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute
This protocol demonstrates the implementation of an optimized N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. A single media formulation is used to reliably obtain healthy brain slices from animals of any age and for diverse experimental applications.
