Accedi

Second Affiliated Hospital of Guangzhou Medical University

2 ARTICLES PUBLISHED IN JoVE

Behavior

Simultaneous Recordings of Cortical Local Field Potentials and Electrocorticograms in Response to Nociceptive Laser Stimuli from Freely Moving Rats

Lupeng Yue^1,2, Fengrui Zhang³, Xuejing Lu^1,2, You Wan^4,5,6, Li Hu^1,2,7

¹CAS Key Laboratory of Mental Health, Institute of Psychology, ²Department of Psychology, University of Chinese Academy of Sciences, ³Research Center of Brain Cognitive Neuroscience, Liaoning Normal University, ⁴Neuroscience Research Institute, Peking University, ⁵Department of Neurobiology, School of Basic Medical Sciences, Peking University, ⁶Key Laboratory for Neuroscience, Ministry of Education/National Health and Family Planning Commission, Peking University, ⁷Department of Pain Management, State Key Clinical Specialty in Pain Medicine, Second Affiliated Hospital of Guangzhou Medical University

We developed a technique that simultaneously records both electrocorticography and local field potentials in response to nociceptive laser stimuli from freely moving rats. This technique helps establish a direct relationship of electrocortical signals at the mesoscopic and macroscopic levels, which facilitates the investigation of nociceptive information processing in the brain.

Cancer Research

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8⁺ T Cells in Mice

Lisha Wang¹, Zhiming Wang¹, Junyi Guo², Huayu Lin¹, Shuqiong Wen², Qiao Liu¹, Yiding Li³, Qing Wu¹, Leiqiong Gao¹, Xiangyu Chen¹, Luoyingzi Xie¹, Qin Tian¹, Jianfang Tang¹, Zhirong Li¹, Li Hu¹, Juan Wang⁴, Lifan Xu¹, Qizhao Huang⁵, Lilin Ye¹

¹Institute of Immunology, Third Military Medical University, ²Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital, Sun Yat-Sen University, ³Shigatse Branch, Xinqiao Hospital, Third Military Medical University, ⁴Department of Emergency Medicine, Southwest Hospital, Third Military Medical University, ⁵Cancer Center, The General Hospital of Western Theater Command

Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses.