Accedi

Beijing Hospital, National Center of Gerontology

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Bibhudatta Mishra¹, Mostafa Ghannad-Rezaie², Jiaxing Li¹, Xin Wang ¹, Yan Hao¹, Bing Ye^3,4, Nikos Chronis^2,5, Catherine A. Collins¹

¹Department of Molecular, Cellular and Developmental Biology, University of Michigan, ²Department of Biomedical Engineering, University of Michigan, ³Life Sciences Institute, University of Michigan, ⁴Department of Cell and Developmental Biology, University of Michigan, ⁵Department of Mechanical Engineering, University of Michigan

Drosophila larvae are an attractive model system for live imaging due to their translucent cuticle and powerful genetics. This protocol describes how to utilize a single-layer PDMS device, called the 'larva chip' for live imaging of cellular processes within neurons of 3^rd instar Drosophila larvae.

Bioengineering

Engineering Platform and Experimental Protocol for Design and Evaluation of a Neurally-controlled Powered Transfemoral Prosthesis

Fan Zhang¹, Ming Liu¹, Stephen Harper^2,3, Michael Lee³, He Huang¹

¹Joint Department of Biomedical Engineering, North Carolina State University & University of North Carolina at Chapel Hill, ²Department of Physical Medicine and Rehabilitation, University of North Carolina School of Medicine, ³Atlantic Prosthetics & Orthotics, LLC

Neural-machine interfaces (NMI) have been developed to identify the user's locomotion mode. These NMIs are potentially useful for neural control of powered artificial legs, but have not been fully demonstrated. This paper presented (1) our designed engineering platform for easy implementation and development of neural control for powered lower limb prostheses and (2) an experimental setup and protocol in a laboratory environment to evaluate neurally-controlled artificial legs on patients with lower limb amputations safely and efficiently.

Medicine

Photoselective Vaporesection of the Prostate via an End-firing Lithium Triborate Crystal Laser

Xin Wang ¹, Yao-Guang Zhang¹, Sheng-Cai Zhu¹, Ben Wan¹, Ming Liu¹, Jian-Ye Wang¹

¹Department of Urology, Beijing Hospital, National Center of Gerontology

Here we present a protocol to perform Photoselective Vaporesection of the Prostate (PVRP) for benign prostatic hyperplasia (BPH) treatment.

Cancer Research

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics

Xin Wang ^*1, Jiamin Zhou^*2, Chen Qi^*1, Gelin Wang^1,3

¹School of Pharmaceutical Sciences, Tsinghua University, ²Department of Radiology, University of California, San Francisco, ³Beijing Advanced Innovation Center for Structural Biology

Establishing a stable cell line overexpressing a gene of interest to study gene function can be done by stable transfection-picking single clones after transfecting them via retroviral infection. Here we show that HT29-DR3 cell lines generated in this way elucidate the mechanisms by which death receptor 3 (DR3) contributes to antimitotics-induced apoptosis.

Medicine

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

Chunlong Fu^*1,2, Yaqun Zhang^*1, Xin Wang¹, Shengcai Zhu¹, Huimin Hou¹, Shengjie Liu¹, Cheng Pang¹, Xinda Song¹, Jia Chen¹, Jianye Wang¹, Ming Liu^1,2

¹(Urology) Beijing Hospital, National Center of Gerontology, The Joint Unit of the Chinese Academy of Medical Sciences, ²Graduate School of Peking Union Medical College, Beijing Hospital

Here, we present a protocol to increase the surgical field of view and reduce the difficulty of total transperitoneal laparoscopic nephroureterectomy surgery by precutting the umbilical ligament before treating the terminal ureter.