Accedi

Kazan Federal University

2 ARTICLES PUBLISHED IN JoVE

Biology

Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy

Gabriele Di Sante¹, Mathew C. Casimiro¹, Timothy G. Pestell², Richard G. Pestell^1,2,3

¹Department of Cancer Biology, Thomas Jefferson University, SKCC, ²Department of Medical Oncology, Thomas Jefferson University, SKCC, ³Kazan Federal University

Herein, we describe in detail a time-lapse video microscopy approach to measuring the temporal recruitment of EYFP-Parkin during the selective removal of damaged mitochondria. This dynamic process of EYFP-Parkin-dependent removal of damaged mitochondria can be used as an indicator of cellular health under different experimental conditions.

Neuroscience

Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction

Dmitry V. Samigullin^1,2,3, Eduard F. Khaziev^1,2,3, Nikita V. Zhilyakov^1,2, Ellya A. Bukharaeva^1,2, Eugeny E. Nikolsky^1,2,4

¹Laboratory of Biophysics of Synaptic Processes, Kazan Scientific Centre, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, ²Open Laboratory of Neuropharmacology, Kazan Federal University, ³Department of Radiophotonics and Microwave Technologies, A.N. Tupolev Kazan National Research Technical University, ⁴Department of Medical and Biological Physics, Kazan State Medical University

Here, we describe a method for loading a calcium-sensitive dye through the frog nerve stump into the nerve endings. We also present a protocol for the recording and analysis of fast calcium transients in the peripheral nerve endings.