Accedi

Beijing Yinfeng Dingcheng Biological Engineering Technology LTD.

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Jianwei Chen^*1,2,3, Ning Shao^*1,2,3, Jiaying Hu^*4, Rong Li⁴, Yuanshou Zhu^1,2,3, Dabing Zhang^4,5, Shujuan Guo^1,2,3, Junhou Hui⁶, Peng Liu⁶, Litao Yang⁴, Sheng-Ce Tao^1,2,3

¹Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, ²State Key Laboratory of Oncogenes and Related Genes, ³School of Biomedical Engineering, Shanghai Jiao Tong University, ⁴Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, ⁵Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, ⁶Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University

This protocol describes the fabrication of a small, ready-to-use cassette that can be applied for visual detection of multiple nucleic acids in a single, test that is easy to operate. In this approach, a capillary array was used for multiplex and highly efficient detection of GMO targets.

Medicine

A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction

Lifei Yang^*1,2, Jianwen Lu^*1,2,3, Yue Wang^1,2,3, Mei Zhang^1,2,3, Yuan Shi⁴, Shasha Wei^1,2, Peng Liu^1,2,3, Zheng Wu³, Yi Lv^1,2,3, Rongqian Wu^1,2

¹Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, First Affiliated Hospital, Xi'an Jiaotong University, ²Institute of Advanced Surgical Technology and Engineering, First Affiliated Hospital, Xi'an Jiaotong University, ³Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi'an Jiaotong University, ⁴Organ Transplantation Center, Tianjin First Center Hospital

The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.

Neuroscience

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats

Peng Liu^*1, Xiu-Chun Song^*2, Xiao-Shuai Yang¹, Qi-Long Cao¹, Yong-Yong Tang¹, Xiao-Dun Liu³, Min Yang¹, Wen-Qiang An¹, Bai-Xiang Dong¹, Xiu-Yun Song¹

¹Beijing Yinfeng Dingcheng Biological Engineering Technology Ltd., ²Hulunbuir People's Hospital, ³Shandong Qilu Stem Cell Engineering Co. Ltd.

The purpose of this study is to establish and validate an animal model for research in the recovery and sequela stages of brain ischemia by testing brain infarction and sensorimotor function after middle cerebral artery occlusion/reperfusion (MCAO/R) after 1-90 days in rats.