Accedi

University of Denver

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Joshua R. Biller^1,2, Deborah G. Mitchell¹, Mark Tseytlin^3,4, Hanan Elajaili¹, George A. Rinard⁵, Richard W. Quine⁶, Sandra S. Eaton¹, Gareth R. Eaton¹

¹Department of Chemistry and Biochemistry, University of Denver, ²Magnetic Imaging Group, Applied Physics Division, Physical Measurements Laboratory, National Institute of Standards and Technology, ³Department of Radiology, Geisel School of Medicine, Dartmouth University, ⁴Department of Biochemistry, West Virginia University, ⁵Department of Electrical and Computer Engineering, University of Denver, ⁶Department of Engineering, University of Denver

A new electron paramagnetic resonance (EPR) method, rapid scan EPR (RS-EPR), is demonstrated for 2D spectral spatial imaging which is superior to the traditional continuous wave (CW) technique and opens new venues for in vivo imaging. Results are demonstrated at 250 MHz, but the technique is applicable at any frequency.

JoVE Journal

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Shawn W. Schowe¹, Conner J. Langeberg², Erich G. Chapman², Kitty Brown³, Marino J. E. Resendiz¹

¹Department of Chemistry, University of Colorado, Denver, ²Department of Chemistry & Biochemistry, University of Denver, ³Analytical Resources Core Bioanalysis & Omics, Colorado State University

MALDI-TOF was used to characterize fragments obtained from the reactivity between oxidized RNA and the exoribonuclease Xrn-1. The present protocol describes a methodology that can be applied to other processes involving RNA and/or DNA.