Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-CAntonia Hauth 1, Rafael Galupa 1, Nicolas Servant 2, Laura Villacorta 1, Kai Hauschulz 3, Joke Gerarda van Bemmel 4, Agnese Loda 1, Edith Heard 1,5
1EMBL: European Molecular Biology Laboratory, 2Institut Curie, 3Agilent Technologies, 4Genmab BV, 5Collège de France
This protocol describes the Capture Hi-C method used to characterize the 3D organization of megabased-sized targeted genomic regions at high-resolution, including boundaries of topologically associating domains (TADs) and long-range chromatin interactions between regulatory and other DNA sequence elements.
