Accedi

Bio-Rad Laboratories, Inc.

2 ARTICLES PUBLISHED IN JoVE

Biology

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Adam M. McCoy¹, Michelle L. Collins¹, Luis A. Ugozzoli¹

¹Gene Expression Division, Bio-Rad Laboratories, Inc.

This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.

Biology

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Adam M. McCoy¹, Claudia Litterst¹, Michelle L. Collins¹, Luis A. Ugozzoli¹

¹Gene Expression Division, Bio-Rad Laboratories

This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.