Accedi

Medical Center, University of California San Francisco

5 ARTICLES PUBLISHED IN JoVE

Biology

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Tobias D. Henning¹, Sophie Boddington¹, Heike E. Daldrup-Link¹

¹Contrast Agent Research Group at the Center for Molecular and Functional Imaging, Department of Radiology, University of California San Francisco

For the evaluation of new stem cell therapies it is important to non-invasively track the injected cells in vivo. This video will show you how to label human mesenchymal and embryonic stem cells with iron oxide based contrast agents in vivo for subsequent MR imaging in vivo.

Biology

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Sophie Boddington¹, Tobias D. Henning¹, Elizabeth J. Sutton¹, Heike E. Daldrup-Link¹

¹Contrast Agent Research Group at the Center for Molecular and Functional Imaging, Department of Radiology, University of California San Francisco

This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.

Biology

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Alexander J. Nedopil¹, Lydia G. Mandrussow¹, Heike E. Daldrup-Link¹

¹Department of Radiology and Biomedical Imaging, Medical Center, University of California San Francisco

Goal of the presentation is to demonstrate a highly reproducible method to generate matrix associated stem cell implants in cartilage defects, which can be visualized with MR imaging. Stem cells are labeled with FDA-approved Ferumoxides, mixed with agarose, implanted into cartilage defects and imaged with a 7T MR scanner.

Medicine

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Rosalinda T. Castaneda^1,2, Aman Khurana^1,2, Ramsha Khan^1,2, Heike E. Daldrup-Link¹

¹Department of Radiology, Molecular Imaging Program at Stanford (MIPS) , ²Stanford School of Medicine, Stanford University

We describe a technique for labeling and tracking stem cells with FDA-approved, superparamagnetic iron oxide (SPIO), ferumoxytol (Feraheme). This cellular imaging technique that utilizes magnetic resonance (MR) imaging for visualization, is readily accessible for long-term monitoring and diagnosis of successful or unsuccessful stem cell engraftments in patients.

Cancer Research

Whole-body PET/MRI of Pediatric Patients: The Details That Matter

Anuj Pareek¹, Anne M. Muehe¹, Ashok J. Theruvath¹, Praveen K. Gulaka¹, Sheri L. Spunt², Heike E. Daldrup-Link¹

¹Department of Radiology, Pediatric Molecular Imaging Program at Stanford (PEDS-MIPS), Stanford University, ²Department of Pediatrics, Stanford University

This article provides comprehensive step-by-step instructions for the acquisition of whole-body 2-deoxy-2-(¹⁸F)fluoro-D-glucose (¹⁸F-FDG) PET/MRI scans for cancer staging of pediatric patients. The protocol was developed for children above 6 years, or old enough to comply with breath-hold instructions, but can be used for general anesthesia patients as well.