Accedi

Tsukuba University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval

Rebecca F. Rosen¹, Yasushi Tomidokoro², Jorge A. Ghiso³, Lary C. Walker^1,4

¹Yerkes National Primate Research Center, Emory University, ²Department of Neurology, Institute of Clinical Medicine, Tsukuba University, ³Department of Pathology, New York University School of Medicine, ⁴Department of Neurology, Emory University

We describe a technique for the preparation of clarified human cortical homogenates, protein separation by SDS-PAGE, antigen retrieval and immunoblotting with an antibody to the Aβ peptide. Using this protocol, we consistently detect monomeric and multimeric Aβ in cortical tissue from humans with Alzheimer's pathology.

Engineering

Detection and Recovery of Palladium, Gold and Cobalt Metals from the Urban Mine Using Novel Sensors/Adsorbents Designated with Nanoscale Wagon-wheel-shaped Pores

Sherif A. El-Safty^1,2, Mohamed A. Shenashen¹, Masaru Sakai³, Emad Elshehy¹, Kohmei Halada¹

¹National Institute for Materials Science, Japan, ²Graduate School for Advanced Science and Engineering, Waseda University, ³Center for Research in Isotopes and Environmental Dynamics, Tsukuba University

Because of the importance and extensive use of palladium, gold and cobalt metals in high-technology equipment, their recovery and recycling constitute an important industrial challenge. The metal recovery system described herein is a simple, low-cost means for the effective detection, removal, and recovery of these metals from the urban mine.