Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway CellsSophie Moreau-Marquis 1, Carly V. Redelman 2, Bruce A. Stanton 1, Gregory G. Anderson 2
1Department of Physiology, Dartmouth College, 2Department of Biology, Indiana University Purdue University Indianapolis
This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.
