Accedi

University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

Janet Alder¹, Wendy Fujioka¹, Jonathan Lifshitz^2,3, David P. Crockett¹, Smita Thakker-Varia¹

¹Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, ²Spinal Cord and Brain Injury Research Center, ³Department of Anatomy and Neurobiology, Department of Physical Medicine and Rehabilitation, University of Kentucky Chandler Medical Center

Lateral fluid percussion (LFP), an established model of traumatic brain injury in mice, is demonstrated. LFP fulfills three major criteria for animal models: validity, reliability and clinical relevance. The procedure, consisting of surgical craniotomy, fixation of hub followed by induction of injury, resulting in focal and diffuse injuries, is described.

Biology

In Ovo Electroporation in Embryonic Chick Retina

Mohammed M. Islam¹, Sung Tae Doh², Li Cai^1,2

¹Department of Pharmacology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, ²Department of Biomedical Engineering, Rutgers University

The overall goal of this video is to show how to perform targeted retinal injection and in ovo electroporation of DNA/RNA constructs into the chick embryonic retina at the Hamburger and Hamilton stage 22-23, which is about embryonic day 4 (E4). This technique is very useful to study gene expression, gene regulation, and morphological change in developing chick retina.