Accedi

Cardiff University

2 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
Xiaoting Meng *1, Wenfei Li *2,3, Fraser Young 1, Runchi Gao 3, Laura Chalmers 3, Min Zhao 3, Bing Song 1
1School of Dentistry, Cardiff Institute of Tissue Engineering & Repair, Cardiff University , 2Shandong Qianfoshan Hospital, Shandong University School of Medicine, 3Dermatology and Ophthalmology Research, Institute for Regenerative Cures, University of California at Davis

This protocol demonstrates methods used to establish 2D and 3D environments in custom-designed electrotactic chambers, which can track cells in vivo/ex vivo using time-lapse recording at the single cell level, in order to investigate galvanotaxis/electrotaxis and other cellular responses to direct current (DC) electric fields (EFs).

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Biology

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
Eleftherios Karanasios 1, Eloise Stapleton 2, Simon A. Walker 1, Maria Manifava 1, Nicholas T. Ktistakis 1
1Signalling Programme, The Babraham Institute, 2MRC Group, Cardiff School of Biosciences, Cardiff University

Time-lapse microscopy of fluorescently labeled autophagy markers allows monitoring of the dynamic autophagy response with high temporal resolution. Using specific autophagy and organelle markers in a combination of 3 different colors, we can follow the contribution of a protein to autophagosome formation in a robust spatial and temporal context.

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