Accedi

Cardiff University

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments

Xiaoting Meng^*1, Wenfei Li^*2,3, Fraser Young¹, Runchi Gao³, Laura Chalmers³, Min Zhao³, Bing Song¹

¹School of Dentistry, Cardiff Institute of Tissue Engineering & Repair, Cardiff University , ²Shandong Qianfoshan Hospital, Shandong University School of Medicine, ³Dermatology and Ophthalmology Research, Institute for Regenerative Cures, University of California at Davis

This protocol demonstrates methods used to establish 2D and 3D environments in custom-designed electrotactic chambers, which can track cells in vivo/ex vivo using time-lapse recording at the single cell level, in order to investigate galvanotaxis/electrotaxis and other cellular responses to direct current (DC) electric fields (EFs).

Biology

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Eleftherios Karanasios¹, Eloise Stapleton², Simon A. Walker¹, Maria Manifava¹, Nicholas T. Ktistakis¹

¹Signalling Programme, The Babraham Institute, ²MRC Group, Cardiff School of Biosciences, Cardiff University

Time-lapse microscopy of fluorescently labeled autophagy markers allows monitoring of the dynamic autophagy response with high temporal resolution. Using specific autophagy and organelle markers in a combination of 3 different colors, we can follow the contribution of a protein to autophagosome formation in a robust spatial and temporal context.