Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow CytometryIris Marangon *1, Nicole Boggetto *2, Cécilia Ménard-Moyon 3, Nathalie Luciani 1, Claire Wilhelm 1, Alberto Bianco 3, Florence Gazeau 1
1Laboratoire Matière et Systèmes Complexes (MSC), CNRS/Université Paris Diderot, 2ImagoSeine BioImaging Core Facility, Institut Jacques Monod, CNRS/Université Paris Diderot, 3Laboratoire d'Immunopathologie et Chimie Thérapeutique, CNRS/Institut de Biologie Moléculaire et Cellulaire
In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.
