High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA VirusesMarianne Lucas-Hourani 1, Hélène Munier-Lehmann 2, Olivier Helynck 2, Anastassia Komarova 1, Philippe Desprès 3, Frédéric Tangy 1, Pierre-Olivier Vidalain 1
1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur
In vitro assays to measure virus replication have been greatly improved by the development of recombinant RNA viruses expressing luciferase or other enzymes capable of bioluminescence. Here we detail a high-throughput screening pipeline that combines such recombinant strains of measles and chikungunya viruses to isolate broad-spectrum antivirals from chemical libraries.
