Accedi

Cincinnati Children's Hospital Medical Center

2 ARTICLES PUBLISHED IN JoVE

Medicine

3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System

Teagan J. Walter^1,2, Erin E. Sparks³, Stacey S. Huppert²

¹Department of Cell and Developmental Biology, Center for Stem Cell Biology, Vanderbilt University, ²Division of Gastroenterology, Hepatology, and Nutrition, Cincinnati Children's Hospital, ³Department of Biology, Duke University

A method of visualizing and quantifying the 3-dimensional structure of mouse hepatic portal vein or intrahepatic bile duct is described. This resin cast technique can also be applied to other ductal or vascular systems and allows for in situ visualization or quantification of a system's intact communicating architecture.

JoVE Journal

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Tony W. Chen^*1, Matthew R. Broadus^*1, Stacey S. Huppert², Ethan Lee^1,3

¹Department of Cell and Developmental Biology and Program in Developmental Biology, Vanderbilt University Medical Center, ²Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, ³Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine

A method is described for analyzing protein degradation using radiolabeled and luciferase-fusion proteins in Xenopus egg extract and its adaptation for high-throughput screening for small molecule modulators of protein degradation.

Cincinnati Children&#39;s Hospital Medical Center

2 ARTICLES PUBLISHED IN JoVE

Medicine

JoVE Journal

Cincinnati Children's Hospital Medical Center