Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatulaSergey Kurdyukov 1, Youhong Song 1, Terence W-Y Tiew 1, Xin-Ding Wang 1, Kim E. Nolan 1, Ray J. Rose 1
1School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales, Australia
The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.
