A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian CellsFarokh Dotiwala 1, Isabelle Fellay 2, Luis Filgueira 2, Denis Martinvalet 3, Judy Lieberman 1, Michael Walch 2
1Cellular and Molecular Medicine Program, Boston Children’s Hospital and Harvard Medical School, 2Department of Medicine, University of Fribourg, 3Centre Médical Universitaire, University of Geneva
We describe here a cost-efficient granzyme expression system using HEK293T cells that produces high yields of pure, fully glycosylated and enzymatically active protease.
