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Howard Hughes Medical Institute, The Rockefeller University

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Despina Soteriou¹, Lana Kostic¹, Egor Sedov¹, Yahav Yosefzon¹, Hermann Steller², Yaron Fuchs¹

¹Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology, ²Strang Laboratory of Apoptosis and Cancer Biology, Howard Hughes Medical Institute, The Rockefeller University

An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.