Accedi

Konkuk University

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Hui Gao¹, Eshita Khera², Jung-Kul Lee¹, Fei Wen²

¹Department of Chemical Engineering, Konkuk University, ²Department of Chemical Engineering, University of Michigan

Presented is the protocol for co-immobilizing whole-cell biocatalysts for cofactor regeneration and improved reusability, using the production of L-xylulose as an example. The cofactor regeneration is achieved by coupling two Escherichia coli strains expressing functionally complementary enzymes; the whole-cell biocatalyst immobilization is achieved by cell encapsulation in calcium alginate beads.

Environment

Cortisol Extraction from Sturgeon Fin and Jawbone Matrices

Jalil Ghassemi Nejad^1,2, Mohammad Ataallahi¹, Mohammad H. Salmanzadeh³, Kyung T. Park³, Hong G. Lee^2,4, Alireza Shoae⁵, Atieh Rahimi⁶, Kyung I. Sung¹, Kyu H. Park¹

¹College of Animal Life Sciences, Kangwon National University, ²Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, ³Persian Gesture, ⁴Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, ⁵Department of Animal Science, Animal Physiology, AgResearch, ⁶Faculty of Agriculture, Animal Science Department, Ferdowsi University of Mashhad

In this study, we present a protocol for cortisol extraction from the fin and jawbone of sturgeon species. Fin and jawbone cortisol levels were further examined by comparing two washing solvents followed by ELISA assays. This study piloted the feasibility of jawbone cortisol as a novel stress indicator.