Accedi

Centre de recherche sur le cancer de l'Université Laval

1 ARTICLES PUBLISHED IN JoVE

Biology

Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments

Margit Fuchs^1,2, Marie-Chloé Boulanger^3,4, Herman Lambert^1,2, Jacques Landry^1,2, Josée N. Lavoie^1,2

¹Département de biologie moléculaire, biochimie médicale et pathologie, Faculté de médecine, Centre de recherche sur le cancer de l'Université Laval, ²Oncology, Centre de recherche du CHU de Québec, Université Laval, ³Laboratoire d'études moléculaires des valvulopathies (LEMV), Groupe de recherche en valvulopathies (GRV), Quebec Heart and Lung Institute/Research Center, ⁴Department of Surgery, Université Laval

We describe a method for depletion-rescue experiments that preserves cellular integrity and protein homeostasis. Adenofection enables functional analyses of proteins within biological processes that rely on finely tuned actin-based dynamics, such as mitotic cell division and myogenesis, at the single-cell level.