Phenotypic Characterization of Macrophages from Rat Kidney by Flow CytometryAlfonso Rubio-Navarro 1, Melania Guerrero-Hue 1, Beatriz Martín-Fernandez 2, Isabel Cortegano 3, Elena Olivares-Alvaro 2, Natalia de las Heras 2, Mario Alía 3, Belén de Andrés 3, María Luisa Gaspar 3, Jesús Egido 1, Juan Antonio Moreno 1
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)
This manuscript describes a detailed protocol for phenotypic and quantitative analysis of resident macrophages from rat kidneys by flow cytometry. The resulting stained cells can be also used for other applications, including cell sorting, gene expression analysis or functional studies, thus increasing the information obtained in the experimental model.
