Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In VitroLena Lavie 1, Larissa Dyugovskaya 1, Andrey Polyakov 1, Oksana Rogovoy 1, Eva Leder 1
1The Lloyd Rigler Sleep Apnea Research Laboratory, Unit of Anatomy and Cell Biology, The Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Insitute of Technology
We describe here a method for obtaining and identifying a newly characterized subpopulation of neutrophil-derived giant phagocytes. These cells develop in culture from fresh human blood neutrophils, and are characterized by phagocytosis, autophagy, immensely large size, and extended lifespan. This method is essential to further investigate this unique neutrophil-derived subpopulation.
