Accedi

University of Texas Long School of Medicine at San Antonio

2 ARTICLES PUBLISHED IN JoVE

Genetics

Methyl-binding DNA capture Sequencing for Patient Tissues

Rohit R. Jadhav¹, Yao V. Wang¹, Ya-Ting Hsu¹, Joseph Liu¹, Dawn Garcia², Zhao Lai², Tim H. M. Huang¹, Victor X. Jin¹

¹Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, ²Greehey Children's Cancer Research Institute, University of Texas Health Science Center at San Antonio

Here we present a protocol to investigate genome wide DNA methylation in large scale clinical patient screening studies using the Methyl-Binding DNA Capture sequencing (MBDCap-seq or MBD-seq) technology and the subsequent bioinformatics analysis pipeline.

Immunology and Infection

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Helia N. Sanchez¹, Tian Shen^1,2, Dawn Garcia^1,3, Zhao Lai^1,3, Paolo Casali¹, Hong Zan¹

¹Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, ²Xiangya School of Medicine, Cental South University, ³Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio

Epigenetic factors can interact with genetic programs to modulate gene expression and regulate B cell function. By combining in vitro B-cell stimulation, qRT-PCR, and high-throughput microRNA-sequence and mRNA-sequence approaches, we can analyze the epigenetic modulation of miRNA and gene expression in B cells.