Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal PlacentaNaimisha Beeravolu 1,2, Christina McKee 1,2, Ali Alamri 1,2, Sasha Mikhael 3, Christina Brown 1,2, Mick Perez-Cruet 2,4, G. Rasul Chaudhry 1,2
1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System
Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.
